Proteome comparison on D. Salexigens cells.
Belarmino A. Salvado Barata, Bruno M. C. Alexandre

D. Salexigens is a salt requiring strict sulfate reducing bacterium, participating in Nature’s sulfur cycling. Under normal conditions, sulfate respiration, a dissimilatory process, yields sulfide coupled to ATP generation and growth (1)
A series of metaloenzymes have been described as involved in the sulfate reduction process and a debate still remains towards the generation of intermediate oxysulfur compounds (2).
The present work compares growth under tiosulfate respiration and their proteome signatures. Optimal conditions for its detection and analysis were looked for and performed.
The cells were either grown under iron constant concentration with or without the presence of other metals, in order to visualize the proteins that are commonly expressed under these respiration modes.
Melanie’s 2.2 analysis of the different gels enabled us to assign different protein patterns responsible for the tiosulfate respiration and their individual assignment and recognition is currently being performed (3).
1)- Postgate, J.R., The Sulphate-reducing bacteria, 2nd Ed., Cambridge University Press, UK, 1984
2)- Methods in Enzymology (H. D. Peck, J. LeGall, Editors), Academic Press, San Diego, 1994; Vol. 243.
3)- The Melanie Group, Geneva, Switzerland, 1997